Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
نویسندگان
چکیده
منابع مشابه
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.
Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is referred to one or several internal control genes, which should not fluctuate d...
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Analyses of different plant stressors are often based on gene expression studies. Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive method for the detection of low abundance transcripts. However, a critical point to note is the selection of housekeeping genes as an internal control. Many so-called 'housekeeping genes' are often affected by different stress factors and may not be sui...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2005
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jxb/eri285